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Table 2 Overview of the discussed in situ tests performed for the BN experiment

From: Impact of the electron donor on in situ microbial nitrate reduction in Opalinus Clay: results from the Mont Terri rock laboratory (Switzerland)

Test code

Interval

\({\text{NO}}_{3}^{ - }\)

\({\text{NO}}_{2}^{ - }\)

CH3COO−

H2 (dissolved)

Start date

Time of e− donor pulse

Sterility solution injected

Parallel injection of tracers

INT1_2011

1

1.5

–

–

–

2011-03-16

No pulse injection

Not sterile

Br− and 2H–H2O

INT1_2014

1

15

–

–

2.3

2014-10-01

54 days

Sterile

–

INT1_2015

1

15

–

–

1.6

2015-09-23

48 days

Sterile

Br−

INT2_2011a

2

1.1

0.8

0.8

–

2011-03-16

No pulse injection

Not sterile

Br− and 2H–H2O

INT2_2011b

2

1.1

–

0.3

–

2011-05-31

No pulse injection

Sterile

–

INT2_2013

2

25

–

4

–

2013-11-05

70 days

Sterile

–

  1. The indicated concentrations (in mM) of nitrate, nitrite, acetate and dissolved H2 are the final concentrations after injection and dilution in the interval